| dc.rights.license | http://creativecommons.org/licenses/by/4.0 - Atribución | es_MX |
| dc.contributor.author | ANGELICA BERENICE AGUILAR GUADARRAMA | es_MX |
| dc.coverage.spatial | MEX - México | es_MX |
| dc.date | 2022 | |
| dc.date.accessioned | 2023-01-12T16:22:06Z | |
| dc.date.available | 2023-01-12T16:22:06Z | |
| dc.identifier.issn | 1424-8247 | |
| dc.identifier.uri | http://riaa.uaem.mx/handle/20.500.12055/3098 | |
| dc.description | Serjania triquetra is a medicinal plant widely used in traditional medicine for the treatment of urinary tract diseases, renal affections, and its complications. The population can buy this plant in folk markets as a raw material mixed with several herbal remedies or as a health supplement. On the market, two commercial presentations were found for the vegetal material; one had a bulk appearance and the other was marketed wrapped in cellophane bags (HESt-2, HESt-3). Nevertheless, the plant has not been exhaustively investigated and quality control techniques have not been developed. This research aimed to realize a phytochemical study using an authentic, freshly collected sample as a reference for S. triquetra (HESt-1), using the compounds identified. A method for the determination of preliminary chromatographic fingerprinting was developed. Additionally, the vasorelaxant effect from three samples was evaluated with ex vivo rat models. Thus, three hydroalcoholic extracts (HESt-1, HESt-2, and HESt-3) were prepared by maceration. A total of nine compounds were fully identified from HESt-1 after the extract was subjected to open-column chromatography. Seven metabolites were detected by gas chromatography, while ursolic acid (UA) and allantoin were isolated and identified using UPLC-MS and NMR, respectively. Three extracts were analyzed for their chromatographic fingerprint by UPLC-MS. Biological activity was explored by ex vivo rat aorta ring model to evaluate vasorelaxant activity. All extracts showed a vasorelaxant effect in a concentration-dependent and endothelium-dependent manner. S. triquetra vascular activity may be attributed to UA and allantoin compounds previously described in the literature for this activity. | es_MX |
| dc.language | eng - Inglés | es_MX |
| dc.publisher | MDPI Open Access Journals | es_MX |
| dc.relation.ispartof | Pharmaceuticals | es_MX |
| dc.relation.ispartofseries | 1289 | es_MX |
| dc.relation.haspart | 15 | es_MX |
| dc.relation.uri | https://www.mdpi.com/1424-8247/15/10/1289 | es_MX |
| dc.rights | openAccess - Acceso Abierto | es_MX |
| dc.subject | 2 - BIOLOGÍA Y QUÍMICA | es_MX |
| dc.subject.classification | vasorelaxant activity; traditional medicine, ursolic acid; allantoin; NMR; UPLC-MS; CG-MS; blood pressure | es_MX |
| dc.subject.other | 24 - CIENCIAS DE LA VIDA | es_MX |
| dc.title | Chromatographic Techniques and Pharmacological Analysis as a Quality Control Strategy for Serjania triquetra a Traditional Medicinal Plant | es_MX |
| dc.type | article - Artículo | es_MX |
| uaem.unidad | Centro de Investigaciones Químicas (CIQ) - Instituto de Investigación en Ciencias Básicas y Aplicadas (IICBA) - Centro de Investigaciones Químicas (CIQ) - Instituto de Investigación en Ciencias Básicas y Aplicadas (IICBA) | es_MX |
| dc.type.publication | publishedVersion | es_MX |
| dc.audience | researchers - Investigadores | es_MX |
